Bio-One of Raleigh decontamination and biohazard cleaning services


Denver, CO – Bio-One Inc. ranked #381 in Entrepreneur magazine’s 43rd annual Franchise 500®. This is the sixth year in a row that Bio-One has been included in the ranking.

A total of 1,177 companies met the requirements, and each franchise was given a cumulative score based on an analysis of more than 150 data points. The 500 franchises with the highest cumulative scores become the Franchise 500® in ranking order. 

“We’re grateful to be recognized by Entrepreneur for the sixth year in a row,” said Brian Brinegar, President of Bio-One Inc. “The ranking is a direct reflection of our growth, strength of our franchisees, and our ability to consistently deliver care and compassion to our clients.”

Succeeding 12 years of franchising, Bio-One has over 115 offices in more than 40 states with services that include crime and trauma scene cleaning, hoarding remediation, odor removal, sewage backups, and more. In 2021, Bio-One also ranked #1 in Entrepreneur’s Best of the Best for Crime-Scene Cleaning

Brian Brinegar Bio-One Quote Entrepreneur Franchise 500

About Bio-One Inc. 

Bio-One provides high quality decontamination and biohazard cleanup services while treating clients with the privacy and compassion that difficult moments demand. Bio-One services include suicide cleanup, homicide cleanup, hoarding remediation, junk removal, deceased animal recovery, feces removal, and more. Bio-One was first created in 2006 and began franchising in 2010. In 2021, Bio-One joined the Five Star Franchising portfolio. Five Star Franchising is a highly innovative conglomerate of franchisor brands with a mission to ensure its brands are top performers in technology, marketing, branding, and people. For more information about Bio-One, visit us at

Mirror Review published the article "Nick-Anthony Zamucen - A Game-Changer in the Restoration Industry" and we couldn't be prouder! Over the past 15 years, Nick has established a Pizzeria Chain franchise, a home care franchise, a bio-recovery franchise, and BOR. All of which are stories of success!

If you're considering stepping into the Bio-One family, here are our top 5 favorite Nick-Anthony Zamucen quotes from this article and how they relate to owning a Bio-One franchise. 

1.  “They’ve gone through enough, let’s go in there and end their day better than it started.”

This is a core mentality of the Bio-One motto Help First, Business Second. Crime scene cleaning is an industry where you know you are making a positive difference in people’s lives, and you will have the opportunity to help people in their greatest time of need. It's as close to being a hero on a day-to-day basis as you can get. 

2. “One of the greatest enjoyments of business is interrupting the ‘status quo’ in set industries." 

Over twenty years ago, the 'status quo' in crime and trauma scene cleaning was DIY. But after first-hand experience, Nick knew it was time for things to change.

Nick encountered a fellow church member who recently lost her husband after he had sadly taken his own life. After police had finished their inspection, she was left with the traumatizing aftermath from the suicide that occurred. With the mindset of helping a community member in need, Nick visited the home with the church minister and helped clean the biohazard. Soon after, Nick realized similar situations are unfortunately faced by countless individuals throughout the country and world, and with the simple idea of helping others, Bio-One was born. 

Fast forward to today, Bio-One Inc. is still paving the way in this industry. In 2021, Bio-One was named number one in crime scene cleaning and trauma scene cleaning in Entrepreneur's Franchise 500 list. Why? Our experienced training and support team will help you launch and build your franchise business. Bio-One has developed the necessary referral networks, created top tier marketing plans, and established vendor relationships to increase your profitability.

3. “You don’t have to wear every hat and figure out every detail by yourself. When you buy a franchise, you are buying into a success strategy, a road map, and a proven game plan." 

One of the benefits of buying into a franchise is that you can be in business for yourself, but not by yourself. Once you begin your Bio-One business, there is a robust training and support program, systems and tools. We begin with our initial franchise training at our headquarters, and ongoing training classes are hosted throughout the year via live webinars and conference calls.

4. “This is fun and business is challenging, but how fun would it be if it were easy? I live to win and compete. I don’t live my life aiming for the second prize. It’s first or nothing…that’s what keeps me motivated."

In order to expect your new business to be a success, prepare to work. You can't just throw a bunch of seeds into the wind and expect a high yield crop. You have to be willing to put in effort from sun up to sun down to get where you want to go. 

Bio-One provides the training and game plan, but the fun begins when you push yourself to execute all you've learned. You will have an incredible support system behind you, but you are the key to your own success. Once you buy a Bio-One franchise, prepare to work like never before. And prepare to be rewarded like never before. 

5. “We teach life skills and business ownership. There is no one size fits all plan for everything, because everyone is uniquely different and needs different things.”

Supporting franchise owners is the backbone of our success. We recognize that Bio-One owners have unique skill sets and business backgrounds. Whether you're a retired police officer, military service member, marketing professional, or data analyst - there's a place for you. 

If you answer "Yes" to any of these questions, Bio-One is a good fit for you!

  • Do you want to build a business that can change your life? 
  • Do you want to be in business for yourself, but not by yourself? 
  • Do you want to own an exciting company where no two days are the same?  
  • Do you want to have the satisfied feeling that comes from helping others while keeping your community safe?

Join The Bio-One Crime Scene Cleaning Franchise Team!


Bio-One Inc. once again was ranked higher then the year prior, up 40 spots to be exact. This is one of the largest jumps in the franchising world and the largest jump in Bio-One Inc.’s history. This is quite an honor and privilege to be named to such a prestigious list. Entrepreneur Magazine only chooses the best of the Best to be listed in its annual Franchise 500 publication.   

Company President, Jason O’Brien, Vice President of Operations Sandi Ellis and Founder Nick-Anthony Zamucen are humbled and full of gratitude to Entrepreneur Magazine for the inclusion and historic increase in position. “We respect and understand the magnitude of the decision to raise our company, Bio-One Inc. to such a high level. To say we our honored would be an understatement.” Said company President Jason O’Brien in a recent interview.  

Bio-One Inc. with the help of their more then 80 franchise offices nationwide, continue to dominant the Crime & Trauma Scene Cleaning and Hoarding space. These nonstop rankings and national awards prove their grip-hold of the market. In addition, Bio-One Inc. is the only Crime & Trauma Scene Cleaning company to ever be ranked in the Entrepreneur Franchise 500.      

Bio-One Inc. would like to thank Entrepreneur Magazine and its staff for believing in the Bio-One Inc. brand, franchise model and their commitment to ongoing excellence.  

Bio-One Inc. has been franchising for eight years and has won more then 30 national awards for excellence, growth, minority opportunity, and commitment to the environment. As the leader in the Crime & Trauma Scene industry they continue to set the standard for professionalism, ethics and integrity in an industry once thought of as ambulance chasers.  Bio-One Inc. has put into place a successful franchise model that not only turned the industry on its head, but transformed the way police, fire, FBI and the US Department of Justice looked at the world of crime scene cleaning

Through hard work, incredibly high standards and a mission to truly Help First and do the business second, Bio-One Inc. continues to innovate, support and lead from the front as their successful franchise grows monthly. If you would like to join our Bio-One family, please don’t hesitate to contact us at 303-625-6543. We are always looking for the best and the brightest franchise owners and operators. Help First, Business Second is not only our company motto, but it is our way of life.  

For more information, please contact: 

Sandi Ellis

Vice President of Franchise Operations  


Bio-One Inc.

5231  S. Quebec St

Suite: 250

Greenwood Village, CO 80111


Bio-One ranked #344 in the franchise 500.Entrepreneur Magazine named us as one of the Best Franchises in the nation for 2017. 

Here at Bio-One we believe in the motto: Help First, Business Second. Bio-One has the opportunity to help people in their greatest time of need and we are proud to make a difference. It's as close to being a hero on a day to day basis as you can get.

We are proud to be the country's #1 Crime and Trauma Scene Cleanup franchise. 

Call us to become part of the Bio-One Team